Fig. 5
Adipocytes enhance breast cancer cells proliferation and migration rate. a MDA-MB-231GFP cell proliferation co-cultured with or without lean or obese 3T3-L1 adipocytes (three independent experiments performed in quadruplicate). b IncuCyte and c MTT assessment of MDA-MB-231 cell proliferation cultured in conditioned media from lean or obese 3T3-L1 adipocytes (three independent experiments performed in quadruplicate). d, e Migration of MDA-MB-231 cells co-cultured with or without lean or obese 3T3-L1 adipocytes (three independent experiments performed in quadruplicate). f MCF-7GFP cell proliferation co-cultured with or without lean or obese 3T3-L1 adipocytes (three independent experiments performed in quadruplicate). g MTT assessment of MCF-7 cell proliferation cultured in conditioned media from lean or obese 3T3-L1 adipocytes (three independent experiments performed in quadruplicate). Data are presented as mean ± SEM. *P ≤ 0.05 vs. controls; #P ≤ 0.05 vs. lean a–d and f, g by two-way ANOVA repeated measure followed by Tukey’s multiple comparisons test and e by one-way ANOVA followed by Tukey’s multiple comparisons test