Fig. 4
[U-13C]-Glucose tracer model data and flux results. Panc-1 cells were cultured in the presence of 40 ng/ml TNFα or 10 ng/ml TGFβ or both for 72 h prior to the start of the labelling experiment. a Metabolic model used for fitting of the extracellular enrichment data, allowing for the reversible exchange of pyruvate and lactate. G6P glucose 6-phosphate, G3P glyceraldehyde 3-phosphate. b Estimated rates of lactate production and uptake of non-glucose substrates from extracellular enrichment data. c Estimated yields of pyruvate dehydrogenase (PDH) and NADP-dependent malic enzyme (ME1) from intracellular enrichment data. Box-and-whisker plots show the 2.5th, 25th, 50th, 75th, and 97.5th percentile values; optimum values shown as asterisks. d Enrichment fractions of intracellular metabolites pyruvate (PYR), lactate (LAC), alanine (ALA), malate (MAL), aspartate (ASP), α-ketoglutarate (AKG), and citrate (CIT) showing the fractions of carbon atoms of these metabolites derived from glucose