Fig. 7
From: A rapid method for quantifying free and bound acetate based on alkylation and GC-MS analysis
Quantification of (sub)cellular total acetate. a Schematic indicating what fractions were analyzed. b Western-blot showing quality of separation of the nuclear (TBP) and residual cellular fraction (tubulin). c Total acetate in whole-cell extract or nuclear and residual cellular fractions. d Same as (c), but expressed relative to protein amount in each fraction. e Histones (red spheres) are heavily acetylated and acetylation controls gene expression. f Western blot of histones following acidic extraction, stained with Ponceau S. g Amount of total acetate from acid extracted histones or nuclear fractionation. h Effect of HDAC inhibitor panobinostat on levels of acetate bound to histones. Values are mean ± SD (n = 3)