Fig. 2From: Bridging the gap between non-targeted stable isotope labeling and metabolic flux analysisWorkflow for non-targeted mass isotopolome analysis. a Non-targeted approaches are valuable for hypothesis generation. These hypotheses are subsequently tested by more targeted techniques leading to refined hypotheses and biological insights. b Data-driven analysis of non-targeted stable isotope labeling data. After stable isotope labeling experiments active pathways and changed fluxes can be detected in a non-targeted manner from metabolome-wide MIDs and changes therein. Compound identity and additional biochemical knowledge is only needed for further interpretation. Analysis of MID similarity between compounds can aid their identification or help to determine their biosynthetic pathways. Yellow boxes highlight new analysis techniques presented in this articleBack to article page