Fig. 3

Low serum conditions induce de novo lipogenesis and alter the composition of cellular free fatty acids. a Incorporation of ¹³C-carbon from glucose, glutamine or acetate in full (FS) or low (LS) serum in DU145 cells. b Quantitative lipid profiling of FFA content of DU145 cells grown in full (FS) or low (LS) serum for 48 h using LC-MS/MS. Data represent the mean ± SEM of three biologically independent replicates. Statistical comparisons were performed using Student t test (*p ≤ 0.05)