Fig. 2
From: Evidence that 2-hydroxyglutarate is not readily metabolized in colorectal carcinoma cells
Strategy for tracking the fate of 2HG. a Successful synthesis of U-13C 2HG is supported by accurate mass measurements and tandem mass spectra. b Metabolomic platform for unbiased tracking of isotopes. Cells were grown in parallel cultures, with and without labeled substrate (n = 3 per group). Metabolites were extracted from the cells and analyzed by LC/MS. The data were processed by using our in-house X13CMS software, which is designed to unbiasedly find differences in isotopic enrichment. These differences correspond to labeled compounds that are possible biotransformations of the labeled substrate provided. Here, we show the isotopologue patterns for 2HG, which had an isotopic enrichment of 35 % in labeled samples. Metabolites of interest were identified by accurate mass, tandem mass spectra, and our isoMETLIN database