Figure 6
From: Quantification of folate metabolism using transient metabolic flux analysis
Glycine accumulation. (a) Glycine synthesis and consumption. Abbreviations: SHMT+, forward SHMT; GCS-, reverse GCS; mlthf, 5,10-methylene-tetrahydrofolate. (b) Glycine concentration 8 h after plating in untreated (open bars) and MTX-treated (filled bars) cells. (c) Estimated glycine exchange rate after corrected for GSH turnover. Positive values indicate uptake, and negative values release. (d) Glycine consumption for protein synthesis. (e) Net SHMT rate (forward minus backward). (f) Forward SHMT rate. (g) Backward SHMT rate. (h) Glycine production from unknown sources. (i) Exchange rate of choline. Positive values indicate uptake and negative values release. Bars represent the average and error bars the standard deviation over three replicates. The symbol *X denotes a difference between untreated and MTX-treated with a statistical significance less than 5 × 10−X.