Figure 5
From: Quantification of folate metabolism using transient metabolic flux analysis
Serine balance. (a) Serine synthesis and consumption. Abbreviations: glc, glucose; ser, serine; gly, glycine; SHMT-, reverse SHMT. (b) Serine concentration 8 h after plating in untreated (open bars) and MTX-treated (filled bars) cells. (c) Serine uptake rate. (d) Serine consumption by protein synthesis. (e) Serine synthesis rate from glucose. (f) 3-phosphoglycerate (3PG) concentration. (g-i) Growth inhibition by co-treatment with MTX (1 to 100 nM) and the PKM2 activator TEPP-46 (100 μM). Bars represent the average and error bars the standard deviation over three replicates. The symbol *X denotes a difference between untreated and MTX-treated with a statistical significance less than 5 × 10−X.