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Figure 1 | Cancer & Metabolism

Figure 1

From: Separation of metabolic supply and demand: aerobic glycolysis as a normal physiological response to fluctuating energetic demands in the membrane

Figure 1

Changes in proton production rates, indicating glycolytic activity, and oxygen consumption rates, corresponding to oxidative phosphorylation, following stimulation/inhibition of membrane transporters. Changes in proton production rates are represented by red diamonds and oxygen consumption rates by blue circles. (A) Stimulation of Na+/K+-ATPase through increasing concentration of Na+/K+-ionophore Gramacidin A in normal human mammary epithelial cells (HMEC), and in dysplastic (MCF10A), non-metastatic (MCF7) and invasive, malignant (MDA-mb-231) cells results in increasing glycolytic activity and little change in oxidative phosphorylation except for a decline at the highest dose. (B) Stimulation of P-glycoprotein (PGP) pumps on the membrane of PGP-expressing MCF7 cells by Verapamil also results in increase of glycolytic activity. (C) Inhibition of membrane activity by increasing concentrations of Ouabain in both malignant (MDA-mb-231) and normal (HMEC) cells results in a decrease in glycolytic rates but no significant change in oxidative metabolism. Error bars represent SD (n = 6).

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Cancer & Metabolism

ISSN: 2049-3002