Effects of leucine supplementation on energy responsive signals of Panc02 tumor cells. (A-C) Western blot analysis of phosphorylated AMPK, ACC, mTOR, p70S6K, and S6 after 20 minutes of 0.3 mM leucine administration after pretreatment with respective media for 3 hours. Data shown are representative blots from three biological replicates, and images for each protein are from the same blot. (B-C) Relative phosphorylation of p-AMPK, p-ACC, p-mTOR, p-p70S6K, and p-S6 in cells grown in media with either (A) 10% FBS or (B) 1% FBS with or without leucine supplementation (* = P <0.05). All data are presented as the mean ± SEM. Differences are considered significant if P <0.05.