Figure 1

Effects of calorie restriction (CR) and/or leucine (LEU) supplementation on body composition glucose tolerance, and hormones. (A) Caloric intake and (B) body weight of C57BL/6 male mice on control and CR diets with and without leucine supplementation reported until glucose tolerance test (GTT) and quantitative magnetic resonance imaging (qMRI) performed (21 weeks, n = 22/group; P <0.001 between groups with different letters). (C) qMRI quantification of body fat and (D) lean mass between mice fed control or CR diets with and without leucine supplementation for 21 weeks (n = 10/group; P <0.001 between all groups with different letters). (E) GTT performed after 21 weeks on diet (n = 10/group; P <0.05 between control and CR, P <0.001 between all other groups with different letters). (F) Fasting glucose levels after 21 weeks on diet (prior to tumor injection; control, n = 14; all other groups, n = 15) (P <0.001 between all groups with different letters). (G-J) Serum hormone analyses after 21 weeks on diet (prior to tumor injection) of (G) insulin (P <0.05 between control and CR + LEU), (H) IGF-1 (P <0.001 between groups with different letters), (I) leptin (P <0.001 between control and both CR groups; P <0.01 between control + LEU and both CR groups), and (J) adiponectin (P <0.001 between CR and both control groups; P <0.05 between all other groups with different letters) (control groups, n = 9; CR groups, n = 10). All data are presented as the mean with error bars indicating the SD (A,B) or SEM (C-J). Differences are considered significant if P <0.05. Abbreviations: CON, control diet; CR,calorie restriction diet; LEU, leucine-supplemented diet.