Figure 2
Sdhb knockdown in mouse ovarian cancer cells leads to EMT. (A) Western blot detection of SDHB, E-cadherin, and TWIST2 after shRNA-mediated knockdown of Sdhb. β-actin was used as a loading control. (B) SDH assay illustrating the decrease in SDH activity upon knockdown of Sdhb. (C) Cell proliferation with counts taken at indicated time points. C1-scr is in black, while C1-sh1-Sdhb and C1-sh2-Sdhb are in red. Ratios are relative to C1-scr at the specified time point. (D) Formation of colonies in soft agar. (E) Bright field cell morphology and immunofluorescent visualization of E-cadherin. (F) Quantification of Snai1, Snai2, Twist1, and Twist2 mRNA by qRT-PCR. Gapdh was used as a control. Statistical significance is based upon student t-test. Experiments were performed in triplicate and repeated at least three times. *p value < .01.