Fig. 2

Differences in OCR and ECAR between MCF7^WT and MCF7^TAM−R. A Mito stress test of MCF7^WT and MCF7^TAM−R cells with oligomycin (Oligo), FCCP and antimycin A/rotenone shows a decreased OCR for MCF7^TAM−R. B Basal, maximal (Max) and spare respiratory capacity (SRC) are decreased in MCF7^TAM−R cells. C Basal OCR with increasing doses of tamoxifen. D Glyco stress test of MCF7^WT and MCF7^TAM−R cells with glucose, oligomycin (Oligo), and 2-DG shows an increased ECAR for MCF7^TAM−R. E Basal and maximal (Max) ECAR are increased in MCF7^TAM−R cells. F Maximal ECAR with increasing concentrations of 4OHT in MCF7^WT and MCF7.^TAM−R cells. Data are represented as mean ± SEM (A, D) and mean + SEM (B, C, E, F; n = 5). Statistical significance was determined by Student’s t test comparing WT with TAM-R (B, E) and with ANOVA with Sidak post hoc test (C, F). ****p < 0.0001