Table 5 Glycolysis, pentose phosphate pathway, serine synthesis, and folate cycle fluxes

J₀: Glc to HxP

7.78±0.26 ^a

23.1±1.0 ^b

16.6±0.5 ^c

20.5±0.1 ^d

Glycolysis

J_2a: HxP to TrP

5.78±0.20 ^a

17.8±1.1 ^b

15.1±0.2 ^c

17.4±0.4 ^b,c

J_2b: TrP to 3PG

11.1±0.4 ^a

35.4±2.2 ^b

29.8±0.4 ^c

33.5±0.9 ^b,c

J_2c: 3PG to Pyr

10.6±0.5 ^a

35.4±2.2 ^b

28.8±0.4 ^c

31.3±1.1 ^b,c

J₄: Pyr to Lac

9.84±0.43 ^a

31.7±2.0 ^b

28.6±0.5 ^b

31.8±1.1 ^b

J_6a: NADH to Mitos

1.82±0.12 ^a

3.70±0.17 ^b

1.98±0.38 ^a

0±0 ^c

J_6b: NADH to Other

0±0 ^a

0±0 ^a

0.19±0.19 ^a

3.86±0.45 ^b

Ox and NonOx PPP

J₁: HxP to R5P

1.12±0.15 ^a

4.76±0.55 ^b

0.81±0.22 ^a

0.33±0.06 ^a

J_3f: R5P to HxP/TrP

2.38±0.32 ^a

0.53±0.06 ^b

0.47±0.13 ^b

1.78±0.17 ^a

J_3r: HxP/TrP to R5P *

3.71±0.50 ^a

1.35±0.16 ^b

1.54±0.42 ^b

5.85±0.56 ^c

Net J₃: HxP/TrP to R5P *

1.33±0.18 ^a

0.81±0.09 ^a

1.07±0.29 ^a

4.07±0.39 ^b

J₉: R5P to NTs

2.45±0.33 ^a,c

5.57±0.65 ^b

1.89±0.51 ^a

4.40±0.43 ^b,c

Serine synthesis and folate cycle

J₁₉: 3PG to Ser

0.54±0.07 ^a,b

0±0 ^a

1.00±0.27 ^b

2.15±0.21 ^c

J₂₀: Endog Ser to FC

1.27±0.17 ^a

4.55±0.52 ^b

0.45±0.12 ^a

1.21±0.12 ^a

J₂₁: Ser to Gly

1.96±0.27 ^a,c

4.46±0.52 ^b

1.51±0.41 ^a

3.52±0.35 ^b,c

J₂₂: Ser exported

0.06±0.00 ^a

0.09±0.00 ^b

0.04±0.00 ^c

0.03±0.00 ^d

J₂₃: Gly consumers

13.9±1.9

nd

7.52±0.2.05

9.43±0.93

J₂₄: Endog Gly to FC

12.1±1.6 ^a

nd

6.11±1.66 ^b

6.10±0.60 ^b

J₂₅: Gly to Ser

0.20±0.03 ^a

nd

0.10±0.03 ^b

0.19±0.02 ^a,b

J₈: NADPH consumers

4.37±0.56 ^a

14.4±1.6 ^b

3.41±0.79 ^a

5.27±0.34 ^a