Breast cancer cells that preferentially metastasize to lung or bone are more glycolytic, synthesize serine at greater rates, and consume less ATP and NADPH than parent MDA-MB-231 cells

Table 3 Simulated ¹³C 3-phosphoglycerate labeling with [1,2-¹³C] glucose

	Predicted 3PG ¹³C isotopologue content (%)
	M	M1	M2	M3
T47D	51.31±0.08	2.07±0.11	46.51±0.19	0.11±0.01
MDA231	50.87±0.15	0.78±0.08	48.35±0.22	<0.01±0.00
BoM	51.03±0.14	1.35±0.16	47.58±0.30	0.05±0.02
LM	50.96±0.05	1.47±0.05	47.50±0.11	0.07±0.00

Experiments with [1,2-¹³C] glucose were conducted as detailed in Table 1. ¹³C 3-phosphoglycerate (3PG) labeling was determined from Model 1 results (Table 1). Relative isotopomer contents (not shown) were used to assess relative folate cycle fluxes (Table 2) to account for the difference between 3PG and serine labeling. Data are mean ± SEM of six experiments per cell line

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