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Fig. 9 | Cancer & Metabolism

Fig. 9

From: Breast cancer cells that preferentially metastasize to lung or bone are more glycolytic, synthesize serine at greater rates, and consume less ATP and NADPH than parent MDA-MB-231 cells

Fig. 9

De novo synthesis of fatty acids and glutathione from glucose and/or glutamine. Cells were pretreated 3–4 h with 40 μM C75 to inhibit fatty acid synthesis or equivalent volume of vehicle (100% ethanol) (A, B). The decrease in glucose or glutamine consumption relative to controls was used to assess flux of these substrates to fatty acids as detailed in Supplemental Information Eq. 12 and 13. Cells were pretreated 2 h with 50 μM BSO to inhibit GSH synthesis or equivalent volume of vehicle (water) (C) and the decrease in glutamine uptake used to assess flux to GSH as detailed in Supplemental Information Eq. 11. Data are mean ± SEM of three to four experiments. Bars sharing common letters or having no letters do not significantly differ (1-way ANOVA p-values for effect of cell line: A, p=0.20; B, p=0.01; C, p=0.29)

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Cancer & Metabolism

ISSN: 2049-3002