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Fig. 7 | Cancer & Metabolism

Fig. 7

From: Breast cancer cells that preferentially metastasize to lung or bone are more glycolytic, synthesize serine at greater rates, and consume less ATP and NADPH than parent MDA-MB-231 cells

Fig. 7

Substrate consumption, lactate production, and protein content for cells metabolizing glucose ± 1.5 mM [5-13C] glutamine. Glucose (A) and glutamine (D) depletion from the media and lactate accumulation (B) was determined over two 1-h intervals as detailed in the methods. Respiration rates (C) of these adherent cells was determined with a Clark-type micro-oxygen electrode as detailed in the “Methods”. SDS-soluble protein content was determined by BCA assay. Data are mean ± SEM of four experiments. Differences between cell lines metabolizing glucose + glutamine were analyzed by 1-way ANOVA with Tukey’s post hoc test. Bars sharing common letters are not significantly different. The effect of glutamine on each cell line was analyzed by paired, two-tailed t-test. * p < 0.05, ** p < 0.01

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