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Fig. 12 | Cancer & Metabolism

Fig. 12

From: Breast cancer cells that preferentially metastasize to lung or bone are more glycolytic, synthesize serine at greater rates, and consume less ATP and NADPH than parent MDA-MB-231 cells

Fig. 12

Estimates of ATP derived from mitochondrial oxidative phosphorylation. A Non-mitochondrial respiration rate was determined as the residual respiration rates measured by Seahorse instrument OCR after addition of 0.5 μM antimycin A and rotenone. Data are mean ± SEM of eight experiments. There was a significant main effect of cell line (1-way ANOVA p = 0.019), but no significant differences by Tukey’s post hoc test. B Oligomycin-sensitive OCR were measured by Seahorse instrument to estimate the percentage of mitochondrial respiration rate used for oxidative phosphorylation. Data are mean ± SD of two experiments. C Mitochondrial and glycolytic ATP synthesis rates for cells metabolizing unlabeled glucose + [5-13C] glutamine were calculated using Eq. 52 and 53 (Supplemental Information) and the percentage ATP derived from oxidative phosphorylation calculated. Data are mean ± SEM of four experiments. Bars sharing common letters are not significantly different

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