Fig. 4

Metabolic pathways affected by high-dose methotrexate (HDMTX) in pediatric acute lymphoblastic leukemia (ALL) patients. A Regularized partial correlation network of the differential metabolites, following HDMTX therapy. Each node represents a compound, and each edge represents the strength of partial correlation between two compounds after conditioning on all other compounds in the dataset. The edge weights represent the partial correlation coefficients. B Bubble chart based on the KEGG pathway analysis of the significantly differential metabolites: (a) pyrimidine metabolism; (b) phenylalanine, tyrosine, and tryptophan biosynthesis; and (c) phenylalanine metabolism