Fig. 5
From: Arginase-1 inhibition reduces migration ability and metastatic colonization of colon cancer cells
ARG1 activity and gene expression are related to malignancy in human colon cancer. A ARG1 protein expression levels in normal (N = 28) and tumor (N = 42) tissues of CRC patients are shown according to the CPTAC database. *P < 0.05 by unpaired t-test. B Arg1 gene expression levels in primary tumor (N = 18) and liver metastasis (N = 18) of CRC patients were analyzed according to the data from the GEO dataset (GSE14297). *P < 0.05 by unpaired t-test. C HCT116 cells (5 × 103) were cultured in the absence and presence of nor-NOHA (0, 125, 250, 500 μM). Living cell numbers were evaluated at 12 h and 24 h. The mean and SD values (n = 4) are indicated. *P < 0.05 by Student’s t-test. D HCT116 cells (2 × 104) were cultured in the absence and presence of nor-NOHA (0, 125, 250, 500 μM). Migration ability was evaluated by Transwell assay at 24 h. Representative images are indicated. Bars represent 200 μM. The mean and SD values (n = 4) are indicated. *P < 0.05 by Student’s t-test