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Fig. 5 | Cancer & Metabolism

Fig. 5

From: Phosphoproteomics revealed cellular signals immediately responding to disruption of cancer amino acid homeostasis induced by inhibition of l-type amino acid transporter 1

Fig. 5

Evaluation of the combined treatment of JPH203 and CX-4945. A Effect of the combination of JPH203 with CX-4945 on cell proliferation. BTC cell lines were treated with JPH203 or CX-4945 alone, or in combination. After 3 days of treatment, cell growth was assessed by WST assay. The absorbance at 450 nm of each sample was expressed as % of the control without JPH203 treatment. B Wound healing assay using KKU-100 treated with 30 μM JPH203 or 5μM CX-4945 alone, or in combination. C The number of migrating cells of KKU-055 and KKU-100 treated with 30 μM JPH203 or 5 μM CX-4945 alone, or in combination. The significance of the difference between the treatment with each inhibitor alone and the combination was determined by one-way ANOVA followed by Tukey’s multiple comparison test. Data represent means ± SD (n = 4 for WST assay, n = 3 for wound healing assay). *Tukey post-hoc p value < 0.05. D Decreased phosphorylation of part of CK2 substrates by LAT1 inhibition with 30 μM JPH203 and 1 μM CX-4945, or in combination for 24 h. Phosphorylation on the consensus CK2 substrate motif was detected by a specific antibody

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Cancer & Metabolism

ISSN: 2049-3002