Fig. 9

SNHG16 promotes 5-Fu resistance through modulating the miR-506-3p-PTBP1-glycolysis axis. a, b AGS 5-Fu-resistant cells were transfected with control, SNHG16 alone, or plus miR-506-3p. Expressions of miR-506-3p and PTBP1 were examined by qRT-PCR and Western blot. c ECAR, d glucose uptake, and e glycolysis enzymes expressions from the above transfected cells were determined. f The above transfected cells were treated with 5-Fu at the indicated concentrations, and cell viabilities were determined by MTT and g Annexin V apoptosis assay. Data were presented as mean±S.D. *p<0.05; **p<0.01; ***p<0.001