Fig. 6

miR-506-3p sensitizes GC cells to 5-Fu by direct targeting PTBP1. a The potential binding of miR-506-3p on PTBP1 3′UTR was predicted from starBase. b AGS and SGC-7901 cells were transfected with control miRNA or miR-506-3p. Protein expressions of PTBP1 were determined. c Luciferase assay was performed in AGS and d SGC-7901 cells transfected with control or miR-506-3p plus WT- or mut-3′UTR of PTBP1. e Pearson coefficient analysis shows a significantly negative correlation between miR-506-3p and PTBP1 in gastric tumors. f AGS and SGC-7901 cells were transfected with control, miR-506-3p alone, or plus PTBP1. Protein expressions of PTBP1 were examined. g, h The glucose uptake and lactate product from the above transfected cells were determined. i, j The above transfected cells were treated with 5-Fu at the indicated concentrations, and cell viability was determined by MTT assay. Data were presented as mean±S.D. *p<0.05; **p<0.01; ***p<0.001