Fig. 5

SNHG16 downregulates miR-506-3p in GC by sponging it. a The potential binding between SNHG16 and miR-506-3p was predicted from starBase. b miR-506-3p expressions were detected in gastric tumors (n=55), and their matched normal gastric tissues by qRT-PCR. c Kaplan-Meier Plotter analyzes the survival rates of GC patients with high or low miR-506-3p expressions. d Pearson coefficient analysis shows a significantly negative correlation between SNHG16 and miR-506-3p in gastric tumors. e Expressions of miR-506-3p were detected in AGS parental and 5-Fu-resistant cells. f, g AGS and SGC-7901 cells were transfected with control miRNA or miR-506-3p. Cells were treated with 5-Fu, and cell viability were determined by MTT assay. h GC cells were transfected with control or SNHG16 siRNA. Expressions of miR-506-3p were detected by qRT-PCR. i Luciferase assay was performed in AGS and SGC-7901 cells transfected with control or miR-506-3p plus WT-SNHG16 or mut-SNHG16. Data were presented as mean±S.D. *p<0.05; **p<0.01; ***p<0.001