Fig. 8

Ketogenic diets reduce metastatic dissemination in the lungs of B16-M4b melanoma allograft mice. A Primary growth of B16-M4b allografts in syngeneic C57BL/6j mice, followed by secondary tumor growth after primary tumor removal (day 11 ± 1; tumor diameter of ~ 1 cm). After surgery, mice were equally assigned to CTRL, LCT or LCT-MCT diet. Mean tumor volume ± SD; n = 8–9. See also Fig. S6. B The area under the growth curve (AUC) was calculated for every mouse. AUCs are shown as individual data points ± SD; p values were determined by a one-way ANOVA with Dunnett’s multiple comparisons test. C Recurrence-free survival of mice fed CTRL, LCT, or LCT-MCT diet. LCT and LCT-MCT survival curves were compared with CTRL, and p values were determined by a log-rank (Mantel-Cox) test. D Blood glucose and E blood beta-hydroxybutyrate (BHB) concentrations of B16-M4b melanoma-bearing mice treated with CTRL, LCT or LCT-MCT on day 8 of dietary intervention. Individual data points ± SD; n = 8–9; p values were determined by a one-way ANOVA with Dunnett's multiple comparisons test, ****p < 0.0001. F Body weight of B16-M4b melanoma-bearing mice during dietary intervention. Net body weight is shown as % of the initial body weight. Individual data points ± SD; n = 8–9. See also Fig. S6. G Percentage of mice presenting metastasis or no metastasis in the lungs of B16-M4b melanoma bearing mice after 14 days of dietary intervention