Fig. 3
From: 13C tracer analysis suggests extensive recycling of endogenous CO2 in vivo
The effect of exogenous NaHCO3 on M + 1 labeling in sarcoma cells. A Schematic of in vitro tracing analysis in media containing NaHCO3 or HEPEPS buffer. B 13C labeling patterns of metabolites from sarcoma cells (in vitro) incubated in [U-13C]-glutamine medium for 3 or 6 h. M + 1/M + 4 ratio (in the insert) was used to account for the overall TCA activity perturbations due to medium changes. Data are presented as mean ± SD of three replicates and after natural abundance correction