Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell lines

Table 1 ATP Production (%) from glycolysis

Cell line	Method	GI_basal	CI	GI_glucose	PI	GI_oligo
HCT116	2D	4.4 ± 3.4%	26.8	31.2 ± 4.3%	67.2	98.4 ± 0.2%
HCT116	3D	55.7 ± 14.8%	1.7	59.4 ± 10.2%	29.7	99.1 ± 1.2%
SW948	2D	3.4 ± 3.3%	52.7	56.1 ± 4.9%	41.8	97.9 ± 0.6%
SW948	3D	47.4 ± 13.5%	10.3	57.7 ± 9.9%	39.7	97.4 ± 1.6%
Panc1	2D	9.0 ± 4.5%	22.5	31.5 ± 4.1%	65.9	97.4 ± 0.5%
Panc1	3D	58.9 ± 17.5%	17.0	75.9 ± 13.7%	23.3	99.2 ± 0.6%
MIA-Pa-Ca-2	2D	17.5 ± 14.6%	28.7	46.2 ± 9.3%	45.3	91.5 ± 6.5%
MIA-Pa-Ca-2	3D	20.7 ± 6.8	19.0	39.7 ± 9.4%	59.0	98.7 ± 0.8%

Glycolytic Index (GI) and bioenergetic phenotypes calculated from Seahorse assay data [30]. GI_basal is before supplementation with glucose, GI_glucose is after supplementation with glucose, and GI_oligo is after injection with oligomycin. Crabtree Index (CI) = GI_glucose – GI_basal. Pasteur Index (PI) = GI_oligo – GI_glucose

ISSN: 2049-3002