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Fig. 4 | Cancer & Metabolism

Fig. 4

From: Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell lines

Fig. 4

ATP production from glycolysis. Percentage of total ATP produced that is attributed to glycolysis was calculated using metrics from Seahorse assays in 2D (blue) and 3D (red) over 2 independent experiments: A HCT116, 2D n = 57 and 3D n = 25. B SW948, 2D n = 55 and 3D n = 47. C Panc1, 2D n = 54 and 3D n = 10. D MIA-Pa-Ca-2, 2D n = 51 and 3D n = 46. Basal is without glucose, glucose is after the addition of glucose, and oligo is after oligomycin injection. Points represent the mean value over all wells, error bars are standard deviation. Statistical significance was calculated by unpaired t test, multiple comparisons (two-stage linear step-up procedure of Benjamin, Krieger and Yekutieli, FDR 1%): p < 0.05: *, p < 0.001: **, p < 0.0001: ***, p < 0.00001: ****

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Cancer & Metabolism

ISSN: 2049-3002