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Fig. 3 | Cancer & Metabolism

Fig. 3

From: Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell lines

Fig. 3

Results from Glycolysis Stress Test assays in 2D and 3D by cell line. Glycolysis Stress Test (GST) traces (left and middle rows) present ECAR data normalized to the ECAR of each sample after glucose injection. Dotted vertical lines indicate injections in the following order: glucose, oligomycin, 2-DG. Metrics from the GST (right row) include basal ECAR without glucose and total glycolytic capacity (maximum ECAR after oligomycin). 2D results are in blue and 3D results are in red. Error bars represent SEM. A HCT116, 2D n = 57 and 3D n = 25. B SW948, 2D n = 55 and 3D n = 47. C Panc1, 2D n = 54 and 3D n = 10. D MIA-Pa-Ca-2, 2D n = 25 and 3D n = 46. Statistical significance was determined using multiple t tests, correction for multiple comparisons by the Holm-Sidak method, with alpha = 0.05. p < 0.05: *, p < 0.001: **, p < 0.0001: ***, p < 0.00001: ****. E ATP-linked respiration (% of basal respiration) vs basal OCR/ECAR, with 3D in red and 3D in blue. Simple linear regression, R2 = 0.7004, with dotted bands showing 95% confidence interval. Pearson correlation = − 0.837, p = 0.0096

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Cancer & Metabolism

ISSN: 2049-3002