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Fig. 2 | Cancer & Metabolism

Fig. 2

From: Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell lines

Fig. 2

Results from Mitochondrial Stress Test assays in 2D and 3D by cell line. A Seahorse set-up for 2D analysis. B Seahorse set-up for 3D analysis. Mitochondrial Stress Test (MST) traces (left and middle rows) present OCR data normalized to the maximum basal value of each sample. Dotted vertical lines indicate injections in the following order: oligomycin, CCCP, rotenone, antimycin A. Metrics from the MST (right row) include ATP-linked respiration (OCR basal max–oligomycin min), total respiratory capacity (maximum OCR after CCCP), complex I activity (OCR CCCP–rotenone) and complex II activity (OCR rotenone–antimycin A). 2D results are in blue and 3D results are in red. Error bars represent SEM. C HCT116, 2D n = 56 and 3D n = 38. D SW948, 2D n = 60 and 3D n = 37. E Panc1, 2D n = 57 and 3D n = 4. F MIA-Pa-Ca-2, 2D n = 54 and 3D n = 47. Statistical significance was determined using multiple t tests, correction for multiple comparisons by the Holm-Sidak method, with alpha = .05. p < 0.05: *, p < 0.001: **, p < 0.0001: ***, p < 0.00001: ****

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Cancer & Metabolism

ISSN: 2049-3002