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Fig. 1 | Cancer & Metabolism

Fig. 1

From: Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell lines

Fig. 1

Cell line proliferation in 2D and spheroid growth. Proliferation of cells in 2D was measured by direct cell counting in a flat-bottom 96-well plate over a period of 5 days, starting cell number of 5000 cells (n = 3 wells): HCT116 (A), SW948 (C), Panc1 (E), MIA-Pa-Ca-2 (G). For spheroid growth measurements, 5000 cells were seeded in different amounts in ultra-low-attachment round bottom plate and then imaged from days 3-8 (n = 10 spheroids, 1 per well): HCT116 (B), SW948 (D), Panc1 (F), MIA-Pa-Ca-2 (H). Measured area is plotted and error bars indicate standard deviation

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Cancer & Metabolism

ISSN: 2049-3002