Fig. 4

Metabolic features and effects of IACS and STN treatments. A, B, C The Seahorse Fuel Flex assay was performed on the A375, A375R1, and UCSD354L cells to determine their dependency (blue) on glucose (GLC), glutamine (GLN), and fatty acids (FA), and their flexibility (orange) to utilize either of the single nutrients when the other two are inhibited. Data are in quadruplicates; error bars, SD. D Seahorse extracellular flux analysis in A375R1 cells. Basal (“B”), oligomycin-inhibited (“O”), FCCP-activated (“F”), and antimycin and rotenone-inhibited (“A&R”) OCR levels were determined after treatments with either vehicle, 100 nM IACS, 1 μM STN, or 1 μM ETMR for 24 h. E Treatment-induced alterations in basal ECAR levels in the cells from the experiment in D. Data represents cell numbers—normalized quadruplicates; and error bars, SD