Fig. 3

In vitro and in vivo efficacy of STN + IACS combinations. A and B A375R1 and UCSD354L cells were treated with IACS (100 nM) or STN (1 μM) or their combinations for 72 h, and cell cycle profiles were generated using propidium iodide–FACS analysis, which included sub-G1 (dead cell) population. C and D Relative cell death induced by the same treatments was confirmed using Cell Death ELISA assay. Data in A–D are in triplicates; error bars, SD. E and F Supervised clustering heatmaps of RPPA-analyzed proteins from A375R1 (E) and UCSD354L (F) that were significantly (p < 0.005) altered after 24 h treatment of cells with IACS, STN, or their combination. The heatmap represents ratio of results from inhibitor-treated versus vehicle-treated samples. Intensity ranges of lowest (blue) and highest (red) protein levels are indicated at the bottom of the heatmaps. G Western blotting of representative proteins and phosphoproteins detected by the RPPA analysis