Fig. 1

C4-2B and PC3 cell cholesteryl ester and growth are influenced by extracellular cholesterol levels but not androgen levels. a Testosterone and b cholesterol levels of fetal calf serum (FCS), charcoal-stripped FCS (CS-FCS), lipoprotein-deficient fetal calf serum (LPDS), and charcoal-stripped LPDS (CS-LPDS). c C4-2B and d PC3 cholesteryl ester levels following 24 h culturing in media supplemented with 10% FCS, 10% CS-FCS, 10% LPDS, or 10% CS-LPDS. e C4-2B cell proliferation in media supplemented with 10% FCS, 10% CS-FCS, 10% LPDS, or 10% CS-LPDS determined by IncuCyte and MTT. f PC3 cell proliferation in media supplemented with 10% FCS, 10% CS-FCS, 10% LPDS, or 10% CS-LPDS determined by IncuCyte and MTT. Data are presented as mean ± SEM of at least three independent experiments performed in triplicate. * P ≤ 0.05 vs. FCS; # P ≤ 0.05 vs. CS-FCS by one-way ANOVA (c and d) or two-way ANOVA (e and f) followed by Tukey’s multiple comparisons test