Fig. 3

MiR-377-3p suppresses proliferation, migration and invasion through inhibition of CPT1C expression in HCC cells. A The proliferation curve of HepG2 cells transfected with CPT1C expression plasmid or miR-377-3p mimics or miR-377-3p mimics plus CPT1C expression plasmid. Western blot analysis shows CPT1C expression. RT-qPCR analysis indicates miR-377-3p expression. B Colony formation assay of HepG2 cells transfected as in A. Graphs at the right panel show relative colony number. Data shown are mean ± SD of 3 independent experiments. C Wound scratch healing assays of HepG2 cells after transfection as in A. Scale bar 50 μm. Histograms show relative cell migration. D, E Transwell assay of HepG2 cells transfected as in A without D or with E Matrigel. Graphs at the right panel show relative cell migration and invasion. The values of the control groups in HepG2 cells were set to 1. Data are mean ± SD (n = 3). **p < 0.01