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Fig. 1 | Cancer & Metabolism

Fig. 1

From: MicroRNA-377-3p inhibits hepatocellular carcinoma growth and metastasis through negative regulation of CPT1C-mediated fatty acid oxidation

Fig. 1

MiR-377-3p targets the 3’-UTR of CPT1C and inhibits its expression in HCC. A HEK-293T cells were transfected with negative control (NC) or mimics of candidate miRNAs as indicated. The representative western blot shows CPT1C expression. β-actin was used as a loading control for western blot. B, C Western blot analysis of CPT1C expression after transfected with (B) NC or miR-377-3p mimics, or (C) scramble or miR-377-3p inhibitor in HepG2 and MHCC97H cells. Histograms show relative CPT1C expression by RT-qPCR. The values of the control groups in HepG2 and MHCC97H cells were set to 1. D Levels of CPT1C mRNA in HepG2 and MHCC97H cells transfected as in (B) and (C). The values of the control groups in HepG2 and MHCC97H cells were set to 1. E Luciferase reporter gene assay was conducted in HepG2 and MHCC97H cells to compare CPT1C 3′-UTR activity among plasmids within wild-type (WT) or mutant (Mut) CPT1C 3′-UTR luciferase reporter with overexpression of miR-377-3p. The top panel indicates WT and Mut forms of putative miR-377-3p target sequences of CPT1C 3′-UTR. Blue font indicates the putative miR-377-3p binding sites within human CPT1C 3′-UTR. Red font indicates the mutations introduced into the CPT1C 3′-UTR. All values shown are mean ± SD (n = 3). **p < 0.01

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