Fig. 5

Mitochondrial function in cells with engineered PDHA1. A Basal mitochondrial (sensitive to 0.3 μM antimycin) OCR of the indicated cells grown in media with only 5 mM glucose as a carbon source. N = 3 biological replicates *P< 0.05, **P< 0.01, ***P< 0.001. B UK5099 (10 μM) sensitive OCR in the same cells and media as in A. N = 3. C Etomixir (68 μM) sensitive OCR in the same cells and media as in A. N = 3. D Remaining mitochondrial OCR after both UK5099 and Etomixir OCR in the same cells and media as in A. N = 3. E PDHA1 KO cell mitochondrial OCR sensitivity after treatment with either glutaminase inhibitor BPTES (8 μM) or transamination inhibitor AOA (1 mM) in the same media as A. N = 3