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Fig. 4 | Cancer & Metabolism

Fig. 4

From: Dynamic regulation of mitochondrial pyruvate metabolism is necessary for orthotopic pancreatic tumor growth

Fig. 4

Addition of 10× TPP and MgCl2 does not rescue enzymatic activity of PDC in PDHA1 mutants. PDC microplate activity assay following immunocapture in standard assay buffer, or assay buffer supplemented with 10× increased TPP (200μM) and MgCl2 (10 mM). Each sample was normalized to the captured PDHA1 as measured by western blot of captured proteins. Cells were treated for 16 h in either 21% or 1% oxygen as indicated. A Standard assay buffer. Kinetic assay results of wilt type and mutant expressing cell lines as indicated. B Reaction buffer with excess thiamine pyrophosphate and magnesium chloride. Kinetic results of wild type and mutant expressing cell lines as indicated. C Endpoint measure from results reported in A and B. Error bars, s.e.m. *P< 0.05

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