Fig. 6
BAM15 reduces tumor growth in vivo by limiting proliferation and mitochondrial function. A Representative H&E (scale = 100 μm), Ki67+ (scale = 100 μm), and TUNEL (scale = 100 μm) staining and quantification of B Ki67+ and C TUNEL staining in tumor sections (CTRL N=4, BAM15 N=5, CR N=5). D ADP stimulated respiration (OXPHOS) and FCCP stimulated respiration (ET) supported by pyruvate, malate, glutamate (N-linked), and E succinate (NS-linked), (CTRL N=5, BAM15 N=7, CR N=5). F FCCP stimulated respiration in the presence of ascorbate/TMPD (Complex IV ET) (CTRL N=5, BAM15 N=7, CR N=5). G Enzymatic activity of citrate synthase (CTRL N=5, BAM15 N=6, CR N=5). H ATP content (CTRL N=5, BAM15 N=6, CR N=5) and I ATP production derived from NS-linked OXPHOS (CTRL N=5, BAM15 N=6, CR N=5). J Hydrogen peroxide (H2O2) activity in tumor homogenates (CTRL N=3, BAM15 N=4, CR N=4). Data are shown as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. Panels B, C, D, E, F, G, H, I, and J were assessed by one-way ANOVA with Tukey’s multiple comparisons. Abbreviations: CTRL, control; CR, calorie restriction; H&E, Hematoxylin and Eosin; Hm, homogenate; PM, pyruvate and malate; D, ADP; G, glutamate; c, cytochrome c; S, succinate; U, uncoupler (FCCP); Rot, rotenone; AmA, antimycin A; As, ascorbate; TMPD, tetramethyl-p-phenylenediamine dihydrochloride; Azd, sodium azide; H2O2, hydrogen peroxide