Fig. 2

BAM15 reduces the expression of genes required for cellular proliferation and energy production in MDA-MB-231 cells. A Principal component analysis of cells exposed to 16 h vehicle or 20 μM BAM15. B Volcano plot illustration of genes differentially expressed by BAM15. C Heat map visualization of top 30 differentially regulated genes. Differentially regulated transcripts were filtered based on the following criteria: q<0.05, base mean>30, and fold change>1.5. D–G Enrichment analysis of canonical signaling pathways differentially regulated by BAM15. Circles (top x-axis) represent the ratio of differentially expressed genes relative to the total number of genes in a given signaling pathway. Bars (bottom x-axis) represent the probability of pathway activation or inactivation based upon differential gene expression patterns. Bar colors represent the directional Z-score generated from pathway enrichment analysis