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Fig. 7 | Cancer & Metabolism

Fig. 7

From: 3-Bromopyruvate-mediated MCT1-dependent metabolic perturbation sensitizes triple negative breast cancer cells to ionizing radiation

Fig. 7

Colony formation assay showing the effects of 3-bromopyruvate (3BP) and ionizing radiation on triple-negative breast cancer cells. (A) MCT1-positive BT20 cells, (B) MCT1-negative MDA-MB-231 cells, and (C) MCT1-knockdown BT20 cells (siMCT1-BT20) were treated with a 50, 100, or 150 μM of 3BP for 1 h, a range of doses of ionizing radiation, or a combination of both where irradiation followed the 3BP treatment. siMCT1-BT20 cells were treated with 3BP and/or radiation and seeded at clonogenic density within 48-h post-transfection. For all groups, n = 6. Statistical significance was assessed with unmatched one-way ANOVA, corrected for multiple comparisons by the Tukey test. Multiplicity adjusted P value is reported. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Unless otherwise marked, star symbols refer to comparisons of each treatment group with the control. Error bars show the standard deviation from the mean

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