Fig. 2
Effects of 3-bromopyruvate (3BP) on glucose metabolism. Changes in (A) glycolysis, (B) pentose phosphate pathway (PPP), and (C) tricarboxylic acid (TCA) cycle in response to 1-h treatment with 100 μM 3BP (n = 6). Please refer to Fig. 3 for a summary of these pathways. Data are expressed as fold change (log2(FC)) compared to untreated controls for each cell line. The heatmaps show the statistical significance (P value < 0.05) of each metabolite change within each cell line. Gray squares in the heatmap indicate missing data. The error bars represent the standard deviation from the mean. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisks, statistical significance of the difference between BT20 cells and each other cell type, unless otherwise shown. Statistical significance was calculated using two-way ANOVA, corrected for multiple comparisons by the Tukey test. The multiplicity adjusted P value is presented