Fig. 2
From: Targeting MYC-enhanced glycolysis for the treatment of small cell lung cancer
PFK158 reduces ATP production and induces apoptosis. A The glycolysis pathway can be inhibited at the major rate-limiting step by targeting PFKFB3 with PFK158. B Validation of MYC, HK2, PFKFB3, and LDHA protein expression in a panel of MYCLow (H1522, H1092, DMS79) and MYCHigh (H446, H82, H524) cell lines. C Calculated IC50 based on ATP luminescence in MYCLow and MYCHigh cell lines. D 11 out of 210 proteins are significantly associated with PFK158 IC50. E cMYC is more highly expressed in cell lines with lower IC50 values. F Annexin V/PI staining in MYCLow cell lines (DMS79, H526, H196, H345, H2196, H1436) and MYCHigh cell lines (H211, H847, H1930, H446, H82, H841) shows percent of apoptotic cells. G Immunoblots of H446 cell line transfected with SCR siRNA, MYC siRNA, and PFKFB3 siRNA showing lower MYC, HK2, PFKFB3, and LDHA protein expression with vinculin loading control. H The percent of viable cells among siRNA-treated H446 cells is not significantly altered. I The percent proliferation cells among siRNA-treated H446 cells is not significantly altered. J ATP luminescence of siRNA knockdown H446 cells after 24 h of 2.5 μM PFK158 treatment. (*P < 0.05; **P < 0.01; ***P < 0.005)