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Fig. 3 | Cancer & Metabolism

Fig. 3

From: Lipopolysaccharide promotes metastasis via acceleration of glycolysis by the nuclear factor-κB/snail/hexokinase3 signaling axis in colorectal cancer

Fig. 3

Snail is required for LPS-induced invasion and migration through P65/inflammasome activation. a The effects of LPS on Snail expression were examined by western blotting. b Scatter plot and spearman correlation analysis of Snail and IL-1β mRNA expression in TCGA database (left) and our data (right) of CRC respectively. Logarithmic transformed data were used in our tissue samples. ρ was Spearman’s rank correlation coefficient. c LPS promoted Snail expression and transportation to the nucleus through inducing inflammasome activation. Cells were pretreated with 3μM caspase-1 inhibitor Ac-YVAD-CHO (cas) for 3 h, and then stimulated with LPS 1μg/ml for 24 h. d Scatter plot and spearman correlation analysis of p65 and snail mRNA expression in TCGA database (left) and our tissue samples (right) of CRC. Logarithmic transformed data were used in our tissue samples. ρ was Spearman’s rank correlation coefficient. e The effects of P65 knockdown on Snail expression were examined by western blotting. Transient siRNA transfections were performed by transfecting 2 × 105 RKO or DLD1 cells with 50nM control siRNA (siNC) or siRNA-P65 for 48 h, then treated with 1μg/ml LPS for 24 h. f Transwell assay was performed to investigate migration and invasion of CRC cells, and quantification of OD value was measured with microplate reader at 570 nm. Established Snail knockdown (shSnail) RKO and DLD1 cell lines were untreated or treated with 1μg/ml LPS. g Transwell assay was performed to investigate migration and invasion of CRC cells, and quantification of OD value was measured with microplate reader at 570 nm. Transient siRNA transfections were performed by transfecting 2 × 106 RKO or DLD1 cells with 50nM control siNC or siRNA-Snail for 48 h, then untreated or treated with 1μg/ml LPS. Data are presented as mean±SEM. *P<0.05, **P<0.01, ***P<0.001. N, nuclear

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