Fig. 5

The effect of 2DG and metformin on the cell cycle and alternative scheduling. a UVW/NAT cells were treated with 3 Gy X-radiation, 1 mM metformin, or 1 mM 2-DG, as single agents or in simultaneous combination for 6 h or 24 h, and cell cycle distribution was determined following propidium iodide staining and flow cytometric analysis. The effect of scheduling on b the cell cycle and c clonogenic survival (n = 4). Data presented are means ± SEM from 3 independent experiments, unless otherwise stated. *p < 0.05, **p < 0.01, ***p < 0.001 compared to untreated control cells