Fig. 1
From: High-resolution spatiotemporal pHe and pO2 imaging in head and neck and oesophageal carcinoma cells
Introduction of the STO2p-Q method. a Schematic representation of STO2p-Q for the measurement of OCR and ECAR. Through lowering of sensor foil-equipped stamps, a micro-respirator is generated facilitating fast measurements of OCR and ECAR. OCR measured using STO2p-Q of FaDu (b) and Kyse30 (c) cells treated with 1.5 μM oligomycin (Oligo) and 1 μM FCCP and DMSO (1:10,000). OCR was normalised by dividing the OCR (decrease of pO2 (%)/time (s)) by the cell number and multiplying with a factor of 107 (normalised OCR). n = 4, from independent experiments. ECAR measured using STO2p-Q of FaDu (d) and Kyse30 (e) cells treated with 3 μM oligomycin and 10 mM glucose (Oligo+Gluc) and 50 mM 2-Deoxy-d-Glucose (2-DG) and DMSO (1:5000). ECAR was normalised by dividing the ECAR (decrease of mpH/time (min)) by the cell number and multiplying with a factor of 104 (normalised ECAR). n = 5, from independent experiments. Data was plotted as dot plots, horizontal black line indicates the mean. Statistical analysis was performed to compare different treatment groups (one-way ANOVA with post hoc Tukey HSD). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001