Fig. 4

TCGA dataset: glutaminolysis- and GSH synthesis-related gene expressions and their correlation in GBM subtypes. a Heatmap representing gene expression levels in G-CIMP, proneural (PN), classical (CS), mesenchymal (MS) GBM subtypes. Upregulated values are in red and downregulated in blue. The RPKM values were normalized by z-score (*p = 0.05, **p < 0.0001, Kruskal-Wallis test). b Box and whiskers plot of GLUD1 and GPT2 expression levels in different GBM subgroups. The lines in the middle of the boxes show the median expression in each group, and the top and the bottom of the boxes represent the first and third quartiles. The results are presented in the log₂ scale of RPKM values. (Kruskal-Wallis: *p < 0.05, **p < 0.005, Dunn test: MS vs PN (α); MS vs CS ($); G-CIMP vs MS: (#). Spearman correlation matrices of gene expression levels in different GBM molecular subtypes. The color bar on the right indicates the level of correlation (r) ranging from dark orange (negative correlation) to purple (positive correlation). The color intensiveness and the circle size are proportional to r values. Only the correlations with p < 0.05 are plotted