Fig. 3
From: Glutaminolysis dynamics during astrocytoma progression correlates with tumor aggressiveness
GLS isoforms expression profile during astrocytoma progression and U87MG human GBM cells. a Western blot analysis of the expression of GLS isoforms in non-neoplastic (NN), pilocytic astrocytoma (AGI), low-grade astrocytoma (AGII), anaplastic astrocytoma (AGIII), glioblastoma (GBM), and U87MG GBM cell line. GLSiso1 and GLSiso2 are indicated by green arrow and red arrows, respectively. β-actin was used as a loading control. b Quantification of the protein relative to β-actin protein by ImageJ, represented by mean values ± standard deviation. The graph is representative of at least two replicates of one experiment. ***p < 0.001, Two-way ANOVA, Bonferroni post-test