Fig. 6
From: Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1
SMAR1 inhibits Warburg effect and tumorigenesis. a Expression of PKM isoforms upon shRNA-mediated knockdown of SMAR1 and PKM2 in MCF7. b % Glucose utilization was measured in shRNA-mediated knockdown of SMAR1 along with PKM2 in MCF7 by enzymatic assay (n = 3). c % Lactate formation was measured in shRNA-mediated knockdown of SMAR1 along with PKM2 in MCF7 by enzymatic assay (n = 3). d % Cell proliferation was measured in shRNA-mediated knockdown of SMAR1 along with PKM2 in MCF7 by MTT assay (n = 3). e % Colony formation was measured in shRNA-mediated knockdown of SMAR1 along with PKM2 in MCF7 (n = 3). f % transwell cell migration was measured in shRNA-mediated knockdown of SMAR1 along with PKM2 in MCF7 (n = 3). g % transwell cell invasion was measured in shRNA-mediated knockdown of SMAR1 along with PKM2 in MCF7 (n = 3). h % Wound migration was measured in shRNA-mediated knockdown of SMAR1 along with PKM2 in MCF7 (n = 3). Error bars show mean values ± SD. Differences were considered statistically significant with *p < 0.05, **p < 0.01 and ***p < 0.001; ns non-significant difference (p > 0.05)