Fig. 5
From: Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1
SMAR1-HDAC6 deacetylates PTBP1 and inhibits its binding to PKM pre-mRNA. a The acetylation status of PTBP1 upon SMAR1 knockdown in MCF7 was checked by IP with an anti-acetyl-lysine antibody. b The acetylation status of PTBP1 upon SMAR1 overexpression followed by Tubacin (5 μM) treatment in MCF7 was checked by IP with the anti-acetyl-lysine antibody. c CLIP experiment of PTBP1 on intron 8 of PKM pre-mRNA in SMAR1 knockdown condition (n = 3). d IP of PTBP1 to confirm pull down in CLIP experiment upon SMAR1 knockdown in MCF7. Error bars show mean values ± SD. Differences were considered statistically significant with *p < 0.05, **p < 0.01, and ***p < 0.001; ns non-significant difference (p > 0.05)